Whole publication list:

  1. (161) Talens-Perales D., J.A. Daròs, J. Polaina and J. Marín-Navarro (2025). Synergistic enzybiotic effect of a bacteriophage endolysin and an engineered glucose oxidase against listeria. Biomolecules, 15 (1): 24, DOI: 10.3390/biom15010024
  2. (160) Lozano-Sanchez E., J.A. Daròs and F. Merwaiss (2024). Production of plant virus-derived hybrid nanoparticles decorated with different nanobodies. ACS Nano, 18 (50): 33890-33906, DOI: 10.1021/acsnano.4c07066
  3. (159) Eiras M., V. Aragonés, J. Marqués, M.D. Gómez and J.A. Daròs (2024). Eggplant latent viroid is located in the chloroplasts and nuclei of eggplant infected cells. Virology Journal, 21 (1): 254, DOI: 10.1186/s12985-024-02530-8
  4. (158) Morote L., A. Rubio-Moraga, A.J. López Jiménez, V. Aragonés, G. Diretto, O. Dermutas, S. Frusciante, O. Ahrazem, J.A. Daròs and L. Gómez-Gómez (2024). Verbascum species as a new source of saffron apocarotenoids and molecular tools for the biotechnological production of crocins and picrocrocin. Plant Journal, 118 (1): 58-72, DOI: 10.1111/tpj.16589
  5. (157) Merwaiss F., E. Lozano-Sanchez, J. Zulaica, L. Rusu, M. Vazquez-Vilar, D. Orzáez, G. Rodrigo, R. Geller and J.A. Daròs (2024). Plant virus-derived nanoparticles decorated with genetically encoded SARS-CoV-2 nanobodies display enhanced neutralizing activity. Plant Biotechnology Journal, 22 (4): 876-891, DOI: 10.1111/pbi.14230
  6. (156) Ortolá B., A. Urbaneja, M. Eiras, M. Pérez-Hedo and J.A. Daròs (2024). RNAi-mediated silencing of Mediterranean fruit fly (Ceratitis capitata) endogenous genes using orally-supplied double-stranded RNAs produced in Escherichia coli. Pest Management Science, 80 (3): 1087-1098, DOI: 10.1002/ps.7839
  7. (155) Ortolá B. and J.A. Daròs (2024). RNA interference in insects: from a natural mechanism of gene expression regulation to a biotechnological crop protection promise. Biology, 13 (3): 137, DOI: 10.3390/biology13030137
  8. (154) Uranga M., V. Aragonés, A. García, S. Mirabel, S. Gianoglio, S. Presa, A. Granell, F. Pasin and J.A. Daròs (2024). RNA virus-mediated gene editing for tomato trait breeding. Horticulture Research, 11 (1): uhad279, DOI: 10.1093/hr/uhad279
  9. (153) J.A. Daròs (2024). Future perspectives in viroid research. In Fundamentals of Viroid Biology, 397-407, DOI: 10.1016/B978-0-323-99688-4.00018-3
  10. (152) Cisneros A.E., T. Martín-García, A. Primc, W. Kuziuta, J. Sánchez-Vicente, V. Aragonés, J.A. Daròs and A. Carbonell (2023). Transgene-free, virus-based gene silencing in plants by artificial microRNAs derived from minimal precursors. Nucleic Acids Research, 51 (19): 10719-10736, DOI: 10.1093/nar/gkad747
  11. (151) Talens-Perales D., M. Nicolau-Sanus, J. Marín-Navarro, J. Polaina and J.A. Daròs (2023). Production in Nicotiana benthamiana of a thermotolerant glucose oxidase that shows enzybiotic activity against Escherichia coli and Staphylococcus aureus. Current Research in Biotechnology, 6: 100148, DOI: 10.1016/j.crbiot.2023.100148
  12. (150) Márquez-Costa R., R. Montagud-Martínez, M.C. Marqués, E. Albert, D. Navarro, J.A. Daròs, R. Ruiz and G. Rodrigo (2023). Multiplexable and biocomputational virus detection by CRISPR-Cas9-mediated strand displacement. Analytical Chemistry, 95 (25): 9564-9574, DOI: 10.1021/acs.analchem.3c01041
  13. (149) Demurtas O.C., M. Sulli, P. Ferrante, P. Mini P, M. Martí, V. Aragonés, J.A. Daròs and G. Giuliano (2023). Production of saffron apocarotenoids in Nicotiana benthamiana plants genome-edited to accumulate zeaxanthin precursor. Metabolites, 13 (6): 729, DOI: 10.3390/metabo13060729
  14. (148) Uranga M. and J.A. Daròs (2022). Tools and targets: the dual role of plant viruses in CRISPR-Cas genome editing. Plant Genome, 16 (2): e20220, DOI: 10.1002/tpg2.20220
  15. (147) Eiras M., A. Mota de Oliveira, A.F. Ramos, R. Harakava and J.A. Daròs (2023). First report of citrus bark cracking viroid and hop latent viroid infecting hop in commercial yards in Brazil. Journal of Plant Pathology, DOI: 10.1007/s42161-023-01313-4
  16. (146) Daròs J.A., F. Pasín and F. Merwaiss (2023). CRISPR-Cas-based plant genome engineering goes viral. Molecular Plant, 16 (4): 660-661, DOI: 10.1016/j.molp.2023.03.010
  17. (145) Uranga M., V. Aragonés, J.A. Daròs and F. Pasín (2023). Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors. Star Protocols, 4 (1): 102091, DOI: 10.1016/j.xpro.2023.102091
  18. (144) Cisneros A.E., P. Lisón, L. Campos, J.M. López-Tubau, T. Altabella, A. Ferrer, J.A. Daròs and A. Carbonell (2023). Downregulation of tomato STEROL GLYCOSYLTRANSFERASE 1 perturbs plant development and facilitates viroid infection. Journal of Experimental Botany, 74 (5): 1564-1578, DOI: 10.1093/jxb/erac361
  19. (143) Ortolá, B. and J.A. Daròs (2023). Viroids: non-coding circular RNAs able to autonomously replicate and infect higher plants. Biology (Basel), 12 (2): 172, DOI: 10.3390/biology12020172
  20. (142) Daròs J.A. (2022). Vectores de expresión derivados de virus de plantasy viroides. Fitopatología, 8: 6-12
  21. (141) Talens-Perales D., M. Nicolau-Sanus, J. Polaina and J.A. Daròs (2022). Expression of an extremophilic xylanase in Nicotiana benthamiana and its use for the production of prebiotic xylooligosaccharides. Scientific Reports, 12 (1): 15743, DOI: 10.1038/s41598-022-19774-5
  22. (140) Selma S., S. Gianoglio, M. Uranga, M. Vázquez-Vilar, A. Espinosa-Ruiz, A. Drapal, P.D. Fraser, J.A. Daròs and D. Orzaez (2022). Potato virus X-delivered CRISPR activation programs lead to strong endogenous gene induction and transient metabolic reprogramming in Nicotiana benthamiana. Plant Journal, 111 (6): 1550-1564, DOI: 10.1111/tpj.15906
  23. (139) Marqués M.C., J. Sánchez-Vicente, R. Ruiz, R. Montagud-Martínez, R. Márquez-Costa, G. Gómez, A. Carbonell, J.A. Daròs and G. Rodrigo (2022). Diagnostics of infections produced by the plant viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13. ACS Synthetic Biology, 11 (7): 2384-2393, DOI: 10.1021/acssynbio.2c00090
  24. (138) Aragonés V., F. Aliaga, F. Pasin and J.A. Daròs (2022). Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors . Biotechnology Journal, 17 (7): e2100504, DOI: 10.1002/biot.202100504
  25. (137) Pasin F., J.A. Daròs and I.E. Tzanetakis (2022). Proteome expansion in the Potyviridae evolutionary radiation. FEMS Microbiology Reviews, 46 (4): fuac011, DOI: 10.1093/femsre/fuac011
  26. (136) Houhou F., M. Martí, T. Cordero, V. Aragonés, C. Sáez, J. Cebolla-Cornejo, A. Pérez de Castro A, M. Rodríguez-Concepción, B. Picó and J.A. Daròs (2022). Carotenoid fortification of zucchini fruits using a viral RNA vector. Biotechnology Journal, 17 (5): e2100328, DOI: 10.1002/biot.202100328
  27. (135) Pallas V., C. Hernández, J.F. Marcos, J.A. Daròs, S. Ambrós, B. Navarro, J.A. Navarro, M. de la Peña, S. Gago-Zachert, M.E. Gas, A. Carbonell, C. Lopez, A.E. Martínez de Alba, F. Di Serio and P. Moreno (2022). In Memoriam of Ricardo Flores: The Career, Achievements, and Legacy of an inspirational plant virologist. Virus Research, 312: 198718, DOI: 10.1016/j.virusres.2022.198718
  28. (134) Rodriguez-Concepcion M. and J.A. Daròs (2022). Transient expression systems to rewire plant carotenoid metabolism. Current Opinion in Plant Biology, 66: 102190, DOI: 10.1016/j.pbi.2022.102190
  29. (133) Martí M., F. Merwaiss, A. Butkovic and J.A. Daròs (2022). Production of potyvirus-derived nanoparticles decorated with a nanobody in biofactory plants. Frontiers in Bioengineering and Biotechnology, 10: 877363, DOI: 10.3389/fbioe.2022.877363
  30. (132) Calvache C., M. Vazquez-Vilar, S. Selma, M. Uranga, A. Fernández-del-Carmen, J.A. Daròs and D. Orzáez (2022). Strong and tunable anti-CRISPR/Cas activities in plants. Plant Biotechnology Journal, 20 (2): 399-408, DOI: 10.1111/pbi.13723
  31. (131) López-Dolz L, M. Spada, J.A. Daròs and A. Carbonell (2022). Fine-tuning plant gene expression with synthetic trans-acting small interfering RNAs. Methods in Molecular Biology (Plant Gene Silencing), 2408: 227-242, DOI: 10.1007/978-1-0716-1875-2_15
  32. (130) Ortolá B. and J.A. Daròs (2022). Production of recombinant RNA in Escherichia coli using eggplant latent viroid as a scaffold. Methods in Molecular Biology (Viroids: Methods and Protocols), 2316: 315-327, DOI: 10.1007/978-1-0716-1464-8_25
  33. (129) Daròs J.A. (2022). Two-dimensional polyacrylamide-gel electrophoresis analysis of viroid RNAs. Methods in Molecular Biology (Viroids: Methods and Protocols), 2316: 79-88, DOI: 10.1007/978-1-0716-1464-8_8
  34. (128) Ortolá B., T. Cordero, X. Hu and J.A. Daròs (2021). Intron-assisted, viroid-based production of insecticidal circular double-stranded RNA in Escherichia coli. RNA Biology, 18 (11): 1846-1857, DOI: 10.1080/15476286.2021.1872962
  35. (127) Uranga M., M. Vazquez-Vilar, D. Orzáez and J.A. Daròs (2021). CRISPR-Cas12a genome editing at the whole-plant level using two compatible RNA virus vectors. CRISPR Journal, 4 (5): 761-769, DOI: 10.1089/crispr.2021.0049
  36. (126) Ambrós S., N. Gómez-Muñoz, S. Giménez-Santamarina, J. Sánchez-Vicente, J. Navarro-López, F. Martínez, J.A. Daròs and G. Rodrigo (2021). Molecular signatures of silencing suppression degeneracy from a complex RNA virus. PLoS Computational Biology, 17 (6): e1009166, DOI: 10.1111/pbi.13723
  37. (125) Montagud-Martínez R., M. Heras-Hernández, L. Goiriz, J.A. Daròs and G. Rodrigo (2021). CRISPR-mediated strand displacement logic circuits with toehold-free DNA. ACS Synthetic Biology, 10 (5): 950-956, DOI: 10.1021/acssynbio.0c00649
  38. (124) Uranga M., V. Aragonés, S. Selma, M. Vázquez-Vilar, D. Orzáez and J.A. Daròs (2021). Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector. Plant Journal, 106 (2): 555-565, DOI: 10.1111/tpj.15164
  39. (123) Daròs J.A. (2021). Identification and functional characterization of viroid circular RNAs. Methods in Molecular Biology (Plant Circular RNAs: Methods and Protocols), 2362:101-107, DOI: 10.1007/978-1-0716-1645-1_6
  40. (122) Daròs J.A. (2021). Production of circular recombinant RNA in Escherichia coli using viroid scaffolds. Methods in Molecular Biology (RNA Scaffolds: Methods and Protocols), 2323: 99-107, DOI: 10.1007/978-1-0716-1499-0_8
  41. (121) Llorente B., S. Torres-Montilla, L. Morelli, I. Florez-Sarasa, J.T. Matus, M. Ezquerro, L. D'Andrea, F. Houhou, E. Majer, B. Picó, J. Cebolla, A. Troncoso, A.R. Fernie, J.A. Daròs and M. Rodriguez-Concepcion (2020). Synthetic conversion of leaf chloroplasts into carotenoid-rich plastids reveals mechanistic basis of natural chromoplast development. Proceedings of the National Academy of Sciences of the USA 117 (35): 21796-21803, DOI: 10.1073/pnas.2004405117
  42. (120) Martí M., G. Diretto, V. Aragonés, S. Frusciante, O. Ahrazem, L. Gómez-Gómez and J.A. Daròs (2020). Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system. Metabolic Engineering 61: 238-250, DOI: 10.1016/j.ymben.2020.06.009
  43. (119) Flores R., J.A. Daròs, C. Hernández, B. Navarro, F. Di Serio (2020). Viroids. Encyclopedia of Life Science, 1: 192-203, DOI: 10.1002/9780470015902.a0029141
  44. (118) López-Dolz L., M. Spada, J.A. Daròs and A. Carbonell (2020). Fine-tune control of targeted RNAi efficacy by plant artificial small RNAs. Nucleic Acids Research 48 (11): 6234-6250, DOI: 10.1093/nar/gkaa343
  45. (117) El-Abeid S.E., Y. Ahmed, J.A. Daròs and M.A. Mohamed (2020). Reduced graphene oxide nanosheet-decorated copper oxide nanoparticles: a potent antifungal nanocomposite against Fusarium root rot and wilt diseases of tomato and pepper plants. Nanomaterials 10 (5): 1001, DOI: 10.3390/nano10051001
  46. (116) Nuraini L., Y. Ando, K. Kawai, F. Tatsuzawa, K. Tanaka, M. Ochiai, K. Suzuki, V. Aragonés, J.A. Daròs and T. Nakatsuka (2020). Anthocyanin regulatory and structural genes associated with violet flower color of Matthiola incana. Planta 251 (3): 61, DOI:10.1007/s00425-020-03351-z
  47. (115) Daròs J.A. (2020). Use of potyvirus vectors to produce carotenoids in plants. Methods in Molecular Biology (Plant and Food Carotenoids) 2083: 303-312, DOI:10.1007/978-1-4939-9952-1_23
  48. (114) Cervera-Seco L., M.C. Marques, A. Sanz-Carbonell, J. Marquez-Molins, A. Carbonell, J.A. Daròs and G. Gomez (2019). Identification and characterization of a stress-responsive TAS3-derived tasiRNAs in melon. Plant and Cell Physiology, 60 (11): 2382-2393, DOI: 10.1093/pcp/pcz131
  49. (113) Carbonell A., P. Lisón and J.A. Daròs (2019). Multi-targeting of viral RNAs with synthetic trans-acting small interfering RNAs enhances plant antiviral resistance. Plant Journal, 100 (4): 720-737, DOI: 10.1111/tpj.14466
  50. (112) Pérez-de-Castro A., C. Esteras, A. Alfaro-Fernández, J.A. Daròs, A.J. Monforte, B. Picó and M.L. Gómez-Guillamón (2019). Fine mapping of wmv1551, a resistance gene to Watermelon mosaic virus in melon. Molecular Breeding, 39 (7): 93, DOI: 10.1007/s11032-019-0998-z
  51. (111) Shi X., T. Cordero, S. Garrigues, J.F. Marcos, J.A. Daròs and M. Coca (2019). Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Plant Biotechnology Journal, 17 (6): 1069-1080, DOI: 10.1111/pbi.13038
  52. (110) Pasin F., W. Menzel and J.A. Daròs (2019). Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses. Plant Biotechnology Journal, 17 (6): 1010-1026, DOI: doi: 10.1111/pbi.13084
  53. (109) Di Serio F., E.M. Torchetti, J.A. Daròs and B. Navarro (2019). Reassessment of viroid RNA cytosine methylation status at the single nucleotide level. Viruses, 11 (4): E357, DOI: 10.3390/v11040357
  54. (108) Gobatto D., L.A. de Oliveira, D.A. de Siqueira Franco, N. Velásquez, J.A. Daròs and M. Eiras (2019). Surveys in the chrysanthemum production areas of Brazil and Colombia reveal that weeds are potential reservoirs of chrysanthemum stunt viroid. Viruses, 11 (4): E355, DOI: 10.3390/v11040355
  55. (107) Aragonés V., A. Pérez-de-Castro, T. Cordero, J. Cebolla-Cornejo, C. López, B. Picó and J.A. Daròs (2019). A Watermelon mosaic virus clone tagged with the yellow visual maker phytoene synthase facilitates scoring infectivity in melon breeding programs. European Journal of Plant Pathology, 153: 317-323, DOI: 10.1007/s10658-018-01621-x
  56. (106) Daròs J.A. (2019). ¿Pueden transformarse los virus de plantas en herramientas útiles para la biotecnología? Revista Alimentaria, 501: 90-92
  57. (105) Carbonell A., C. López and J.A. Daròs (2019). Fast-forward identification of highly effective artificial small RNAs against different tomato spotted wilt virus isolates. Molecular Plant-Microbe Interactions, 32 (2): 142-156, DOI: 10.1094/MPMI-05-18-0117-TA
  58. (104) Carbonell A. and J.A. Daròs (2019). Design, synthesis, and functional analysis of highly specific artificial small RNAs with antiviral activity in plants. Methods in Molecular Biology, 2028: 231-246, DOI: 10.1007/978-1-4939-9635-3_13
  59. (103) Cordero T., V. Aragonés and J.A. Daròs (2018). Large-scale production of recombinant RNAs on a circular scaffold using a viroid-derived system in Escherichia coli. Journal of Visualized Experiments, 141: e58472, DOI: 10.3791/58472
  60. (102) Cordero T., A. Rosado, E. Majer, A. Jaramillo, G. Rodrigo and J.A. Daròs (2018). Boolean computation in plants using post-translational genetic control and a visual output signal. ACS Synthetic Biology, 7 (10): 2322-2330, DOI: 10.1021/acssynbio.8b00214
  61. (101) Cordero T., B. Ortolá and J.A. Daròs (2018). Mutational analysis of Eggplant latent viroid RNA circularization by the eggplant tRNA ligase in Escherichia coli. Frontiers in Microbiology 9: 635, DOI: 10.3389/fmicb.2018.00635
  62. (100) Daròs J.A., V. Aragonés and T. Cordero (2018). A viroid-derived system to produce large amounts of recombinant RNA in Escherichia coli. Scientific Reports 8 (1): 1904, DOI: 10.1038/s41598-018-20314-3
  63. (99) Rodrigo G., S. Prakash, S. Shen, E. Majer, J.A. Daròs and A. Jaramillo (2017). Model-based design of RNA hybridization networks implemented in living cells. Nucleic Acids Research 45 (16): 9797-9808, DOI: 10.1093/nar/gkx698
  64. (98) Daròs J.A. (2017). Viral suppressors: combating RNA silencing. Nature Plants 3: 17098, DOI: 10.1038/nplants.2017.98
  65. (97) Carbonell A. and J.A. Daròs (2017). Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection. Molecular Plant Pathology 18 (5): 746-753, DOI: 10.1111/mpp.12529
  66. (96) Abdel-Hafez S.I.I., N.A. Nafady, I.R. Abdel-Rahim, A.M. Shaltout, J.A. Daròs and M.A. Mohamed (2017). Biosynthesis of silver nanoparticles using the compound curvularin isolated from the endophytic fungus Epicoccum nigrum: characterization and antifungal activity. Journal of Pharmaceutical and Applied Chemistry 3 (2): 135-146, DOI: 10.18576/jpac/030207
  67. (95) López-González S., V. Aragonés, J.A. Daròs, F. Sánchez and F. Ponz (2017). An infectious cDNA clone of a radish-infecting Turnip mosaic virus strain. European Journal of Plant Pathology 148: 207-211, DOI: 10.1007/s10658-016-1057-9
  68. (94) Cordero T., M.A. Mohamed, J.J. López-Moya and J.A. Daròs (2017). A recombinant Potato virus Y infectious clone tagged with the Rosea1 visual marker (PVY-Ros1) facilitates the analysis of viral infectivity and allows the production of large amounts of anthocyanins in plants. Frontiers in Microbiology 8: 611, DOI: 10.3389/fmicb.2017.00611
  69. (93) Rodrigo G., J.A. Daròs and S.F. Elena (2017). Virus-host interactome: putting the accent on how it changes. Journal of Proteomics 156: 1-4, DOI: 10.1016/j.jprot.2016.12.007
  70. (92) Majer E., B. Llorente, M. Rodríguez-Concepción and J.A. Daròs (2017). Rewiring carotenoid biosynthesis in plants using a viral vector. Scientific Reports 7: 41645, DOI: 10.1038/srep41645
  71. (91) Cordero T., Cerdán L., Carbonell A., Katsarou K., Kalantidis K. and J.A. Daròs (2017). Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana. Molecular Plant-Microbe Interactions 30 (1): 63-71, DOI: 10.1094/MPMI-11-16-0239-R
  72. (90) Daròs J.A. (2017). Eggplant latent viroid. In Viroids and satellites (editors A. Hadidi, R. Flores, J.W. Randles and P. Palukaitis), Oxford: Academic Press, pp. 339-344. ISBN: 978-0-12-801498-1, Elsevier Inc.
  73. (89) Abdel-Hafez S.I.I, N.A. Nafady, I.R. Abdel-RAhim, A.M. Shaltout, J.A. Daròs and M.A. Mohamed (2016). Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani. 3 Biotech 6 (2): 199, DOI: 10.1007/s13205-016-0515-6
  74. (88) Daròs J.A. (2016). Eggplant latent viroid: a friendly experimental system in the family Avsunviroidae. Molecular Plant Pathology 17 (8):1170-1177, DOI: 10.1111/mpp.12358
  75. (87) Willemsen A., M.P. Zwart, N. Tromas, E, Majer, J.A. Daròs and S.F. Elena (2016). Multiple barriers to the evolution of alternative gene orders in a positive-strand RNA virus. Genetics 202 (4): 1503-1521, DOI: 10.1534/genetics.115.185017
  76. (86) Martínez F., G. Rodrigo, V. Aragonés, M. Ruiz, I. Lodewijk, U. Fernández, S.F. Elena and J.A. Daròs (2016). Interaction network of tobacco etch potyvirus NIa protein with the host plant during infection. BMC Genomics 17: 87, DOI: 10.1186/s12864-016-2394-y
  77. (85) Carbonell A., J.C. Carrington and J.A. Daròs (2016). Fast-forward generation of effective artificial small RNAs for enhanced antiviral defense in plants. RNA & Disease 3 (1): e1130. DOI: 10.14800/rd.1130
  78. (84) Daròs, J.A. (2016). Viroids: small noncoding infectious RNAs with the remakable ability of autonomous replication. En Current Research Topics in Plant Virology (editores Aiming Wang and Xueping Zhou). Springer International Publishing Switzerland, pp. 295-322. ISBN: 978-3-319-32917-8, ISBN: 978-3-319-32919-2 (eBook), DOI: 10.1007/978-3-319-32919-2_13.
  79. (83) Rodrigo G., E. Majer, S. Prakash, J.A. Daròs, A. Jaramillo and J.F. Poyatos (2015). Exploring the dynamics and mutational landscape of riboregulation with a minimal synthetic circuit in living cells. Biophysical Journal 109 (5): 1070-1076, DOI: 10.1016/j.bpj.2015.07.021
  80. (82) Majer E., J.A. Navarro and J.A. Daròs (2015). A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein. Biotechnology Journal 10 (11): 1792-1802, DOI: 10.1002/biot.201500042
  81. (81) Debreczeni D.E., C. López, J. Aramburu, J.A. Daròs, S. Soler, L. Galipienso, B.W. Falk and L. Rubio (2015). Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain. Archives of Virology 160 (8): 2117-2123, DOI: 10.1007/s00705-015-2453-8
  82. (80) Shen S., G. Rodrigo, S. Prakash, E. Majer, T.E. Landrain, B. Kirov, J.A. Daròs and A. Jaramillo (2015). Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleics Acids Research 43 (10): 5158-5170, DOI: 10.1093/nar/gkv287
  83. (79) Martínez F. and J.A. Daròs (2014). Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection. Journal of Virology 88 (18): 10725-10737, DOI: 10.1128/JVI.00928-14
  84. (78) Majer E., Z. Salvador, M.P. Zwart, A. Willemsen, S.F. Elena and J.A. Daròs (2014). Relocation of the NIb gene in the tobacco etch potyvirus genome. Journal of Virology 88 (8): 4586-4590, DOI: 10.1128/JVI.03336-13
  85. (77) Gobatto D., A.L.R. Chaves, R. Harakava, J.M. Marque, J.A. Daròs and M. Eiras (2014). Chrysanthemum stunt viroid in Brazil: survey, identification, biological and molecular characterization and detection methods. Journal of Plant Pathology 96 (1): 111-119.
  86. (76) Zwart M.P., A. Willemsen A., J.A. Daròs, S.F. Elena (2014). Experimental evolution of pseudogenization and gene loss in a plant RNA virus. Molecular Biology Evolución 31 (1): 121-134, DOI: 10.1093/molbev/mst175
  87. (75) Majer E., J.A. Daròs and M.P. Zwart (2013). Stability and fitness impact of the visually discernible Rosea1 marker in the Tobacco etch virus genome. Viruses 5 (9): 2153-2168, DOI: 10.3390/v5092153
  88. (74) Rodrigo G., T.E. Landrain, E. Majer, J.A. Daròs and A. Jaramillo (2013). Full design automation of multi-state RNA devices to program gene expression using energy-based optimization. PLoS Computational Biology 9 (8): e1003172, DOI: 10.1371/journal.pcbi.1003172
  89. (73) Martínez F., S.F. Elena and J.A. Daròs (2013). Fate of artificial microRNA-mediated resistance to plant viruses in mixed infections. Phytopathology 103 (8): 870-876, DOI: 10.1094/PHYTO-09-12-0233-R
  90. (72) Lafforgue G., F. Martínez, Q.W. Niu, N.H. Chua, J.A. Daròs and S.F. Elena (2013). Improving the effectiveness of amiRNA-mediated resistance against Turnip mosaic virus by combining two amiRNAs or by targeting highly conserved viral genomic regions. Journal of Virology 87 (14): 8254-8256, DOI: 10.1128/JVI.00914-13
  91. (71) Contreras-Paredes C.A., L. Silva-Rosales, J.A. Daròs, N.D. Alejandri-Ramírez, T.D. Dinkova (2013). The absence of eukaryotic initiation factor eIF(iso)4E affects the systemic spread of a Tobacco etch virus isolate in Arabidopsis thaliana. Molecular Plant-Microbe Interactions 26 (4): 461-470, DOI: 10.1094/MPMI-09-12-0225-R
  92. (70) Martínez F., G. Lafforgue G, M.J. Morelli, F. González-Candelas, N.H. Chua, J.A. Daròs and S.F. Elena (2012). Ultra-deep sequencing analysis of population dynamics of virus escape mutants in RNAi-mediated resistant plants. Molecular Biology Evolution 29 (11): 3297-3307, DOI: 10.1093/molbev/mss135
  93. (69) Zwart M., J.A. Daròs and S.F. Elena (2012). Effects of potyvirus effective population size in inoculated leaves on viral accumulation and the onset of symptoms. Journal of Virology 86 (18): 9737-9747, DOI: 10.1128/JVI.00909-12
  94. (68) Nohales M.A., R. Flores and J.A. Daròs (2012). Viroid RNA redirects host DNA ligase 1 to act as an RNA ligase. Proceedings of the National Academy of Sciences of the USA 109 (34): 13805-13810, DOI: 10.1073/pnas.1206187109
  95. (67) Nohales M.A., D. Molina-Serrano, R. Flores and J.A. Daròs (2012). Involvement of the chloroplastic isoform of tRNA ligase in the replication of viroids belonging to the family Avsunviroidae. Journal of Virology 86 (15): 8269-8276, DOI: 10.1128/JVI.00629-12
  96. (66) Sardanyés, J., F. Martínez, J.A. Daròs and S.F. Elena (2012). Dynamics of alternative modes of RNA replication for positive-sense RNA viruses. Journal of the Royal Society Interface 9 (69): 768-776, DOI: 10.1098/rsif.2011.0471.
  97. (65) Molina-Serrano D., J. Marqués J, M.A. Nohales, R. Flores and J.A. Daròs (2012). A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2’-5’ RNA ligase. RNA Biology 9 (3): 326-333, DOI: 10.4161/rna.19218
  98. (64) Marqués J., N. Duran-Vila, A. Namsi, J.M. Bové and J.A. Daròs (2012). Bacteria associated with the rhizosphere of manganese-deficient date palms affected by brittle leaf disease. Journal of Plant Pathology 94 (1): 157-169.
  99. (63) Bedoya L.C., F. Martínez, D. Orzáez and J.A. Daròs (2012). Visual tracking of plant virus infection and movement using a reporter MYB transcription factor that activates anthocyanin biosynthesis. Plant Physiology 158 (3): 1130-1138, DOI: 10.1104/pp.111.192922
  100. (62) De la Iglesia F., F. Martínez, J. Hillung, J.M. Cuevas, P.J. Gerrish, J.A. Daròs and S.F. Elena (2012). Luria-Delbrück estimation of turnip mosaic virus mutation rate in vivo. Journal of Virology 86 (6): 3386-3388, DOI: 10.1128/JVI.06909-11
  101. (61) Lafforgue, G., F. Martínez, J. Sardanyés, F. de la Iglesia, Q.W. Niu, S.S. Lin, R.V. Solé, N.H. Chua, J.A. Daròs and S.F. Elena (2011). Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance. Journal of Virology 85 (19): 9686-9695, DOI: 10.1128/JVI.05326-11
  102. (60) Zwart, M., J.A. Daròs and S.F. Elena (2011). One is enough: in vivo effective population size is dose-dependent for a plant RNA virus. PLoS Pathogens 7 (7): e1002122, DOI: 10.1371/journal.ppat.1002122
  103. (59) Martínez, F., J. Sardanyés, S.F. Elena and J.A. Daròs (2011). Dynamics of a plant RNA virus intracellular accumulation: stamping machine versus geometric replication. Genetics 188 (3): 637-646, DOI: 10.1534/genetics.111.129114
  104. (58) Marqués, J., N. Duran-Vila and J.A. Daròs (2011). The Mn-binding proteins of the photosystem II oxygen-evolving complex are decreased in date palms affected by brittle leaf disease. Plant Physiology and Biochemistry 49 (4): 388-394, DOI: 10.1016/j.plaphy.2011.02.008
  105. (57) Eiras, M., M.A. Nohales, E.W. Kitajima, R. Flores and J.A. Daròs (2011). Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA. Archives of Virology 156 (3): 529-533, DOI: 10.1007/s00705-010-0867-x
  106. (56) Flores, R., J.A. Daròs, C. Hernández, F. Di Serio (2011). Viroids. Encyclopedia of Life Sciences, DOI: 10.1002/9780470015902.a0000434.pub3.
  107. (55) Bedoya, L.C., F. Martínez, L. Rubio and J.A. Daròs (2010). Simultaneous equimolar expression of multiple proteins in plants from a dissarmed potyvirus vector. Journal of Biotechnology, 150 (2): 268-275, DOI: 10.1016/j.jbiotec.2010.08.006
  108. (54) Eiras, M., S.R. Silva, E.S. Stuchi, R. Flores and J.A. Daròs (2010). Viroid species associated with the bark-cracking phenotype of ‘Tahiti’ acid lime in the State of São Paulo, Brazill. Tropical Plant Pathology 35 (5): 303-309.
  109. (53) Bedoya, L.C. and J.A. Daròs (2010). Stability of Tobacco etch virus infectious clones in plasmid vectors. Virus Research 149 (2): 234-240. DOI: 10.1016/j.virusres.2010.02.004
  110. (52) Torres-Barceló, C., J.A. Daròs and S.F. Elena (2010). Compensatory molecular evolution of HC-Pro, an RNA-silencing suppressor from a plant RNA virus. Molecular Biology and Evolution 27 (3): 543-551, DOI: 10.1093/molbev/msp272
  111. (51) Torres-Barceló, C., J.A. Daròs and S.F. Elena (2010). HC-Pro hypo- and hypersuppressor mutants: differences in viral siRNA accumulation in vivo and siRNA binding activity in vitro. Archives of Virology 155 (2): 251-254, DOI: 10.1007/s00705-009-0563-x
  112. (50) Martínez, F., J. Marqués, M.L. Salvador and J.A. Daròs (2009). Mutational analysis of Eggplant latent viroid RNA processing in Chlamydomonas reinhardtii chloroplast. Journal of General Virology 90 (12): 3057-3065, DOI: 10.1099/vir.0.013425-0
  113. (49) Elena, S.F., G. Gómez and J.A. Daròs (2009). Evolutionary constraints to viroid evolution. Viruses, 1 (2): 241-254, DOI: 10.3390/v1020241
  114. (48) Flores, R., M.E. Gas, D. Molina-Serrano, M.A. Nohales, A. Carbonell, S. Gago, M. de la Peña and J.A. Daròs (2009). Viroid replication: Rolling-circles, enzymes and ribozymes. Viruses, 1 (2): 317-334, DOI: 10.3390/v1020317
  115. (47) Serra, P., M. Eiras, S.M. Bani-Hashemian, N. Murcia, E.W. Kitajima, J.A. Daròs, R. Flores and N. Duran-Vila (2008). Citrus viroid V: occurrence, host range, diagnosis, and identification of new variants. Phytopathology, 98 (11): 1199-1204, DOI: 10.1094/PHYTO-98-11-1199
  116. (46) Torres-Barceló, C., S. Martín, J.A. Daròs and S.F. Elena (2008). From hypo- to hypersuppression: effect of amino acid substitutions on the RNA-silencing suppressor activity of the Tobacco etch potyvirus HC-Pro. Genetics, 180 (2): 1039-1049, DOI: 10.1534/genetics.108.091363
  117. (45) Gas, M.E., D. Molina-Serrano, C. Hernández, R. Flores and J.A. Daròs (2008). Monomeric linear RNA of Citrus exocortis viroid resulting from processing in vivo has 5'-phosphomonoester and 3'-hydroxyl termini: implications for the RNase and RNA ligase involved in replication. Journal of Virology, 82 (20): 10321-10325, DOI: 10.1128/JVI.01229-08
  118. (44) Duran-Vila, N., S.F. Elena, J.A. Daròs and R. Flores (2008). Structure and evolution of viroids. In Origin and evolution of viruses (2nd edition), E. Domingo, C.R. Parrish and J. Holland (eds.) pp. 43-64. Elsevier. ISBN: 978-0-12-374153. DOI: 10.1016/B978-0-12-374153-0.00002-3
  119. (43) Marqués, J., Z.G.N. Fadda, N. Duran-Vila, R. Flores, J.M. Bové and J.A. Daròs (2008). A set of novel RNAs transcribed from the chloroplast genome accumulates in date palm leaflets affected by brittle leaf disease. Phytopathology, 98 (3): 337-344, DOI: 10.1094/PHYTO-98-3-0337
  120. (42) Serra, P., C.J. Barbosa, J.A. Daròs, R. Flores and N. Duran-Vila (2008). Citrus viroid V: molecular characterization and synergistic interactions with other members of the genus Apscaviroid. Virology, 370 (1): 102-112, DOI: 10.1016/j.virol.2007.07.033
  121. (41) Flores, R., M.E. Gas, D. Molina, C. Hernández and J.A. Daròs (2008). Analysis of viroid replication. Methods in Molecular Biology, 451: 167-183.
  122. (40) Gas, M.E., C. Hernández, R. Flores and J.A. Daròs (2007). Processing of nuclear viroids in vivo: an interplay between RNA conformations. PLoS Pathogens, 3 (11): 1813-1826, DOI: 10.1371/journal.ppat.0030182
  123. (39) Martin, R., C. Arenas, J.A. Daròs, A. Covarrubias, J.L. Reyes and N.H. Chua (2007). Characterization of small RNAs derived from Citrus exocortis viroid (CEVd) in infected tomato plants. Virology, 367 (1): 135-146, DOI: 10.1016/j.virol.2007.05.011
  124. (38) Sanjuán, R. and J.A. Daròs (2007). One-step site-directed mutagenesis of viroid dimeric cDNA. Journal of Virological Methods, 145 (1): 71-75, DOI: 10.1016/j.jviromet.2007.05.027
  125. (37) Eiras, M., E.W. Kitajima, R. Flores and J.A. Daròs (2007). Existence in vivo of the loop E motif in potato spindle tuber viroid RNA. Archives of Virology, 152 (7): 1389-1393, DOI: 10.1007/s00705-007-0952-y
  126. (36) Molina-Serrano, D., L. Suay, M.L. Salvador, R. Flores and J.A. Daròs (2007). Processing of RNAs of the family Avsunviroidae in Chlamydomonas reinhardtii chloroplasts. Journal of Virology, 81 (8): 4363-4366, DOI: 10.1128/JVI.02556-06
  127. (35) Carrasco, P., J.A. Daròs, P. Agudelo-Romero and S.F. Elena (2007). A real-time RT-PCR assay for quantifying the fitness of tobacco etch virus in competition experiments. Journal of Virological Methods, 139 (2): 181-188, DOI: 10.1016/j.jviromet.2006.09.020
  128. (34) Namsi, A., J. Marqués, Z. Fadda, M.L. Takrouni, O. Ben Mahamoud, A. Zouba, J.A. Daròs, R. Flores, J.M. Bové and N. Duran-Vila (2006). Diagnosis of “maladie des feuilles cassantes” or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs. Molecular and Cellular Probes, 20 (6): 366-370, DOI: 10.1016/j.mcp.2006.05.001
  129. (33) Codoñer, F.M., J.A. Daròs, R.V. Solé and S.F. Elena (2006). The fittest versus the flattest: experimental confirmation of the quasispecies effect with subviral pathogens. PLoS Pathogens, 2 (12): 1187-1193, DOI: 10.1371/journal.ppat.0020136
  130. (32) Eiras, M., J.A. Daròs, R. Flores and E.W. Kitajima (2006). Viróides e virusóides: relíquias do mundo de RNA. Fitopatologia Brasileira, 31 (3): 229-246, DOI: 10.1590/S0100-41582006000300001
  131. (31) Hernández, C., F. Di Serio, S. Ambrós, J.A. Daròs and R. Flores (2006). An element of the tertiary structure of peach latent mosaic viroid RNA revealed by UV irradiation. Journal of Virology, 80 (18): 9336-9340, DOI: 10.1128/JVI.00630-06
  132. (30) Di Serio, F., J.A. Daròs, A. Ragozzino and R. Flores (2006). Close structural relationship between two hammerhead viroid-like RNAs associated with cherry chlorotic rusty spot disease. Archives of Virology, 151 (8): 1539-1549, DOI: 10.1007/s00705-006-0732-0
  133. (29) Daròs, J.A., S.F. Elena and R. Flores (2006). Viroids: an Ariadne’s thread into the RNA labyrinth. EMBO Reports, 7 (6): 593-598, DOI: 10.1038/sj.embor.7400706
  134. (28) Elena, S.F., P. Carrasco, J.A. Daròs and R. Sanjuán (2006). Mechanisms of genetic robustness in RNA viruses. EMBO Reports, 7 (2): 168-173, DOI: 10.1038/sj.embor.7400636
  135. (27) Flores, R., J.A. Daròs, C. Hernández and F. Di Serio (2005). Viroids. En Encyclopedia of Life Sciences, S. Robertson (ed.). Macmillan Reference Limited (Nature Publishing Group), London, U.K. www.els.net
  136. (26) Flores, R., C. Hernández, A.E. Martínez de Alba, J.A. Daròs and F. Di Serio (2005). Viroids and viroid-host interactions. Annual Reviews in Phytopathology, 43: 117-139, DOI: 10.1146/annurev.phyto.43.040204.140243
  137. (25) Daròs, J.A. and R. Flores (2004). Arabidopsis thaliana has the enzymatic machinery for replicating representative viroid species of the family Pospiviroidae. Proceedings of the National Academy of Sciences of the USA, 101 (17):6792-6797, DOI: 10.1073/pnas.0401090101
  138. (24) Fadda, Z., J.A. Daròs, R. Flores and N. Durán-Vila (2003). Identification in eggplant of a variant of citrus exocortis viroid (CEVd) with a 96 nucleotide duplication in the right terminal region of the rod-like secondary structure. Virus Research, 97 (2):145-149, DOI: 10.1016/j.virusres.2003.08.002
  139. (23) Triki, M.A., A. Zouba, O. Khoualdia, O. Ben Mahamoud, M.L. Takrouni, M. Garnier, J.M. Bové, M. Montarone, A. Poupet, R. Flores, J.A. Daròs, Z.G.N. Fadda, P. Moreno and N. Durán-Vila (2003). “Maladie des feuilles cassantes” or brittle leaf disease of date palms in Tunisia: biotic or abiotic disease? Journal of Plant Pathology, 85 (2): 71-79.
  140. (22) Fadda, Z., J.A. Daròs, C. Fagoaga, R. Flores and N. Durán-Vila (2003). Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids). Journal of Virology, 77 (11): 6528-6532, DOI: 10.1128/JVI.77.11.6528-6532.2003
  141. (21) Flores, R., J.A. Daròs and J.A. Navarro (2003). Replication. In Viroids, A. Hadidi, R. Flores, J.W. Randles and J.S. Semancik (eds.) pp. 55-60. CSIRO Publishing, Collingwood VIC3066, Australia. ISBN: 0 643 06789 2.
  142. (20) Daròs, J.A. and R. Flores (2002). A chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage. EMBO Journal, 21 (4): 749-759, DOI: 10.1093/emboj/21.4.749
  143. (19) Flores, R., C. Hernández, M. de la Peña, A. Vera and J.A. Daròs (2001). Hammerhead ribozyme structure and function in plant RNA replication. Methods in Enzymology, 341: 540-552.
  144. (18) Flores, R., J.A. Daròs, C. Hernández and F. Di Serio (2001). Viroids. En Encyclopedia of Life Sciences, S. Robertson (ed.). Macmillan Reference Limited (Nature Publishing Group), London, U.K. www.els.net
  145. (17) Vera, A., J.A. Daròs, R. Flores and C. Hernández (2000). The DNA of a plant retroviroid-like element is fused to different sites in the genome of a plant pararetrovirus and shows multiple forms with sequence deletions. Journal of Virology, 74 (22): 10390-10400, DOI: 10.1128/JVI.74.22.10390-10400.2000
  146. (16) Flores, R., J.A. Daròs and C. Hernández (2000). Avsunviroidae family: viroids containing hammerhead ribozymes. Advances in Virus Research, 55: 271-323, DOI: 10.1016/S0065-3527(00)55006-4
  147. (15) Daròs, J.A., M. Schaad and J.C. Carrington (1999). Functional analysis of the interaction between VPg-proteinase (NIa) and RNA polymerase (NIb) of tobacco etch potyvirus, using conditional and suppressor mutants. Journal of Virology, 73 (10): 8732-8740.
  148. (14) Navarro, J.A., J.A. Daròs and R. Flores (1999) Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization. Virology, 253 (1): 77-85, DOI: 10.1006/viro.1998.9497
  149. (13) Navarro, B., J.A. Daròs and R. Flores (1998). Reverse transcription polymerase chain reaction protocols for cloning small circular RNAs. Journal of Virological Methods, 73 (1): 1-9, DOI: 10.1016/S0166-0934(98)00042-1
  150. (12) Haldeman-Cahill, R., J.A. Daròs and J.C. Carrington (1998). Secondary structures in the capsid protein coding sequence and 3' nontranslated region involved in amplification of the tobacco etch virus genome. Journal of Virology, 72 (5): 4072-4079.
  151. (11) Daròs, J.A. and J.C. Carrington (1997). RNA binding activity of NIa proteinase of tobacco etch potyvirus. Virology, 237 (2): 327-336, DOI: 10.1006/viro.1997.8802
  152. (10) Di Serio, F., J.A. Daròs, A. Ragozzino and R. Flores (1997). A 451-nucleotide circular RNA from cherry with hammerhead ribozymes in its strands of both polarities. Journal of Virology, 71 (9): 6603-6610.
  153. (9) Daròs, J.A. and R. Flores (1996). A group I plant intron accumulates as circular RNA forms with extensive 5’ deletions in vivo. RNA, 2 (9): 928-936.
  154. (8) Navarro, B., J.A. Daròs and R. Flores (1996). A general strategy for cloning viroids and other small circular RNAs that uses minimal amounts of template and does not require prior knowledge of its sequence. Journal of Virological Methods, 56 (1): 59-66, DOI: 10.1016/0166-0934(95)01912-X
  155. (7) Daròs, J.A. y R. Flores (1995). Viroides y otros pequeños RNAs circulares e infecciosos de animales y plantas. En Microbiología y Genética Molecular, Josep Casadesús (ed.) Servicio de Publicaciones de la Universidad de Huelva. ISBN: 84-88751-24-9.
  156. (6) Daròs, J.A. and R. Flores (1995). Characterization of multiple circular RNAs derived from a plant viroid-like RNA by sequence deletions and duplications. RNA, 1 (7): 734-744.
  157. (5) Daròs, J.A. and R. Flores (1995). Identification of a retroviroid-like element from plants. Proceedings of the National Academy of Sciences of the USA, 92 (15): 6856-6860, DOI: 10.1073/pnas.92.15.6856
  158. (4) Daròs, J.A., J.F. Marcos, C. Hernández and R. Flores (1994). Replication of avocado sunblotch viroid: evidence for a symmetric pathway with two rolling circles and hammerhead ribozyme processing. Proceedings of the National Academy of Sciences of the USA, 91 (26): 12813-12817, DOI: 10.1073/pnas.91.26.12813
  159. (3) Hernández, C., J.A. Daròs, S.F. Elena, A. Moya and R. Flores (1992). The strands of both polarities of a small circular RNA from carnation self-cleave in vitro through alternative double- and single-hammerhead structures. Nucleic Acids Research, 20 (23): 6323-6329, DOI: 10.1093/nar/20.23.6323
  160. (2) Braco, L., J.A. Daròs and M. de la Guardia (1992). Enzymatic flow injection analysis in nonaqueous media. Analytical Chemistry, 64 (2): 129-133, DOI: 10.1021/ac00026a006
  161. (1) Rández-Gil, F., J.A. Daròs, A. Salvador and M. de la Guardia (1991). Direct derivative spectrophotometric determination of nitrazepam and clonazepam in biological fluids. Journal of Pharmaceutical and Biomedical Analysis, 9 (7): 539-545, DOI: 10.1016/0731-7085(91)80175-9