See the whole publication list in PubMed or Web of Science .

Some selected publications:

  1. Merwaiss F., E. Lozano-Sanchez, J. Zulaica, L. Rusu, M. Vazquez-Vilar, D. Orzáez, G. Rodrigo, R. Geller and J.A. Daròs (2023). Plant virus-derived nanoparticles decorated with genetically encoded SARS-CoV-2 nanobodies display enhanced neutralizing activity. Plant Biotechnology Journal 10.1111/pbi.14230. PubMed
  2. Ortolá B., A. Urbaneja, M. Eiras, M. Pérez-Hedo and J.A. Daròs (2023). RNAi-mediated silencing of Mediterranean fruit fly (Ceratitis capitata) endogenous genes using orally-supplied double-stranded RNAs produced in Escherichia coli. Pest Management Science DOI: 10.1002/ps.7839. PubMed
  3. Talens-Perales D., M. Nicolau-Sanus, J. Marín-Navarro, J. Polaina and J.A. Daròs (2023). Production in Nicotiana benthamiana of a thermotolerant glucose oxidase that shows enzybiotic activity against Escherichia coli and Staphylococcus aureus. Current Research in Biotechnology 126: 100148. CRB
  4. Uranga M. and J.A. Daròs (2023). Tools and targets: The dual role of plant viruses in CRISPR-Cas genome editing. Plant Genome 16: e20220. PubMed
  5. Daròs J.A., F. Pasin and F. Merwaiss (2023). CRISPR-Cas-based plant genome engineering goes viral. Molecular Plant 16: 660-661. PubMed
  6. Uranga M., V. Aragonés, J.A. Daròs and F. Pasin (2023). Tools and targets: Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors. Star Protocols 4: 102091. PubMed
  7. Ortolá B. and J.A. Daròs (2023). Viroids: non-coding circular RNAs able to autonomously replicate and infect higher plants. Biology 12: 172. PubMed
  8. Talens-Perales D., M. Nicolau-Sanus, J. Polaina and J.A. Daròs (2022). Expression of an extremophilic xylanase in Nicotiana benthamiana and its use for the production of prebiotic xylooligosaccharides. Scientific Reports 12: 15743. PubMed
  9. Marqués M.C., J. Sánchez-Vicente, R. Ruiz, R. Montagud-Martínez, R. Márquez-Costa, G. Gómez, A. Carbonell, J.A. Daròs and G. Rodrigo (2022). Diagnostics of infections produced by the plant viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13. ACS Synthetic Biology 11: 2384-2393. PubMed
  10. Aragonés V., F. Aliaga, F. Pasin and J.A. Daròs (2022). Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors. Biotechnology Journal 17: e2100504. PubMed
  11. Houhou F., M. Martí, T. Cordero, V. Aragonés, C. Sáez, J. Cebolla-Cornejo, A. Pérez de Castro, M. Rodríguez-Concepción, B. Picó and J.A. Daròs (2022). Carotenoid fortification of zucchini fruits using a viral RNA vector. Biotechnology Journal 17: e2100328. PubMed
  12. Martí M.C., F. Merwaiss, A. Butkovic and J.A. Daròs (2022). Production of potyvirus-derived nanoparticles decorated with a nanobody in biofactory plants. Frontiers in Bioengineering and Biotechnology 10: 877363. PubMed
  13. Ortolá B. and J.A. Daròs (2022). Production of recombinant RNA in Escherichia coli using eggplant latent viroid as a scaffold. Methods in Molecular Biology 2316: 315-327. PubMed
  14. Daròs J.A. (2022). Two-dimensional polyacrylamide-gel electrophoresis analysis of viroid RNAs. Methods in Molecular Biology 2316: 79-88. PubMed
  15. Ortolà B., T. Cordero, X. Hu and J.A. Daròs (2021). Intron-assisted, viroid-based production of insecticidal circular double-stranded RNA in Escherichia coli. RNA Biology 18: 1846-1857. PubMed
  16. Uranga M., M. Vazquez-Vilar, D. Orzáz and J.A. Daròs (2021). CRISPR-Cas12a genome editing at the whole-plant level using two compatible RNA virus vectors. CRISPR Journal 4:761-769. PubMed
  17. Uranga M., V. Aragonés, S. Selma, M. Vázquez-Vilar, D. Orzáez and J.A. Daròs (2021). Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector. Plant Journal 106: 555-565. PubMed
  18. Daròs J.A. (2021). Identification and functional characterization of viroid circular RNAs. Methods in Molecular Biology 2362: 101-107. PubMed
  19. Daròs J.A. (2021). Production of circular recombinant RNA in Escherichia coli using viroid scaffolds. Methods in Molecular Biology 2323: 99-107. PubMed
  20. Martí M., G. Diretto, V. Aragonés, S. Frusciante, O. Ahrazem, L. Gómez-Gómez and J.A. Daròs (2020). Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system. Metabolic Engineering 61: 238-250. PubMed
  21. Daròs J.A. (2020). Use of potyvirus vectors to produce carotenoids in plants. Methods in Molecular Biology 2083: 303-312. PubMed
  22. Shi X., T. Cordero, S. Garrigues, J.F. Marcos, J.A. Daròs and M. Coca (2019). Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Plant Biotechnology Journal 17: 1069-1080. PubMed
  23. Aragonés V., A. Pérez-de-Castro, T. Cordero, J. Cebolla-Cornejo, C. López, B. Picó and J.A. Daròs (2019). A Watermelon mosaic virus clone tagged with the yellow visual maker phytoene synthase facilitates scoring infectivity in melon breeding programs. European Journal of Plant Pathology 153: 317-323. EJPP
  24. Di Serio F., E.M. Torchetti, J.A. Daròs and B. Navarro (2019). Reassessment of viroid RNA cytosine methylation status at the single nucleotide level. Viruses 11: E357. PubMed
  25. Daròs J.A. (2019). ¿Pueden transformarse los virus de plantas en herramientas útiles para la biotecnología? Revista Alimentaria, 501: 90-92.
  26. Carbonell A., C. López and J.A. Daròs (2019). Fast-forward identification of highly effective artificial small RNAs against different tomato spotted wilt virus isolates. Molecular Plant-Microbe Interactions 32: 142-156. PubMed
  27. Cordero T., V. Aragonés and J.A. Daròs (2018). Large-scale production of recombinant RNAs on a circular scaffold using a viroid-derived system in Escherichia coli. Journal of Visualized Expriments 141: e58472. PubMed
  28. Cordero T., A. Rosado, E. Majer, A. Jaramillo, G. Rodrigo and J.A. Daròs (2018). Boolean computation in plants using post-translational genetic control and a visual output signal. ACS Synthetic Biology, 7: 2322-2330. PubMed
  29. Cordero T., B. Ortolá and J.A. Daròs (2018). Mutational analysis of Eggplant latent viroid RNA circularization by the eggplant tRNA ligase in Escherichia coli. Frontiers in Microbiology 9: 635. PubMed
  30. Daròs J.A., V. Aragonés and T. Cordero (2018). A viroid-derived system to produce large amounts of recombinant RNA in Escherichia coli. Scientific Reports 8: 1904. PubMed
  31. Daròs J.A. (2017). Viral suppressors: combating RNA silencing. Nature Plants 3: 17098. PubMed
  32. Cordero T., M.A. Mohamed M.A., J.J. López-Moya and J.A. Daròs (2017). A recombinant Potato virus Y infectious clone tagged with the Rosea1 visual marker (PVY-Ros1) facilitates the analysis of viral infectivity and allows the production of large amounts of anthocyanins in plants. Frontiers in Microbiology 8: 611. PubMed
  33. Majer E., B. Llorente, M. Rodríguez-Concepción and and J.A. Daròs (2017). Rewiring carotenoid biosynthesis in plants using a viral vector. Scientific Reports 7: 41645. PubMed
  34. Cordero T., L. Cerdán, A. Carbonell, K. Katsarou, K. Kalantidis and J.A. Daròs (2017). Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana. Molecular Plant-Microbe Interactions 30: 63-71. PubMed
  35. J.A. Daròs (2016). Eggplant latent viroid: a friendly experimental system in the family Avsunviroidae. Molecular Plant Pathology 17: 1170-1177. PubMed
  36. Martínez F., G. Rodrigo, V. Aragonés, I. Lodewijk, U. Fernández, S.F. Elena and J.A. Daròs (2016). Interaction network of tobacco etch potyvirus NIa protein with the host plant during infection. BMC Genomics 17: 87. PubMed
  37. Majer E., J.A. Navarro and J.A. Daròs (2015). A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein. Biotechnology Journal 10: 1792-1802. PubMed
  38. Martínez F. and J.A. Daròs (2014). Tobacco etch virus P1 protein traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection. Journal of Virology 88: 10725-10737. PubMed
  39. Majer E., Z. Salvador, M.P. Zwart, A. Willemsen, S.F. Elena and J.A. Daròs (2014). Relocation of the NIb gene in the tobacco etch potyvirus genome. Journal of Virology 88: 4586-4590. PubMed
  40. Martínez F., S.F. Elena and J.A. Daròs (2013). Fate of artificial microRNA-mediated resistance to plant viruses in mixed infections. Phytopathology 103: 870-876. PubMed
  41. Nohales M.A., R. Flores and J.A. Daròs (2012). Viroid RNA redirects host DNA ligase 1 to act as an RNA ligase. Proceedings of the National Academy of Sciences of the USA 109: 13805-13810. PubMed
  42. Nohales M.A., D. Molina-Serrano, R. Flores and J.A. Daròs (2012). Involvement of the chloroplastic isoform of tRNA ligase in the replication of viroids belonging to the family Avsunviroidae. Journal of Virology 86: 8269-8276. PubMed
  43. Molina-Serrano D., J. Marqués J, M.A. Nohales, R. Flores and J.A. Daròs (2012). A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2’-5’ RNA ligase. RNA Biology 9: 326-333. PubMed
  44. Marqués J., N. Duran-Vila, A. Namsi, J.M. Bové and J.A. Daròs (2012). Bacteria associated with the rhizosphere of manganese-deficient date palms affected by brittle leaf disease. Journal of Plant Pathology 94: 157-169. JPP
  45. Bedoya L.C., F. Martínez, D. and J.A. Daròs (2012). Visual tracking of plant virus infection and movement using a reporter MYB transcription factor that activates anthocyanin biosynthesis. Plant Physiology 158: 1130-1138. PubMed
  46. Martínez F., J. Sardanyés, S.F. Elena and J.A. Daròs (2011). Dynamics of a plant RNA virus intracellular accumulation: stamping machine versus geometric replication. Genetics 188: 637-646. PubMed
  47. Marqués J., N. Duran-Vila and J.A. Daròs (2011). The Mn-binding proteins of the photosystem II oxygen-evolving complex are decreased in date palms affected by brittle leaf disease. Plant Physiology and Biochemistry 49: 388-394. PubMed
  48. Eiras M., M.A. Nohales, E.W. Kitajima, R. Flores and J.A. Daròs (2011). Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA. Archives of Virology, 156: 529-533. PubMed
  49. Bedoya L.C., F. Martínez, L. Rubio and J.A. Daròs (2010). Simultaneous equimolar expression of multiple proteins in plants from a dissarmed potyvirus vector. Journal of Biotechnology 150: 268-275. PubMed
  50. Bedoya L.C. and J.A. Daròs (2010). Stability of Tobacco etch virus infectious clones in plasmid vectors. Virus Research 149: 234-240. PubMed
  51. Martínez F., J. Marqués, M.L. Salvador and J.A. Daròs (2009). Mutational analysis of Eggplant latent viroid RNA processing in Chlamydomonas reinhardtii chloroplast. Journal of General Virology 90: 3057-3065. PubMed
  52. Gas M.E., D. Molina-Serrano, C. Hernández, R. Flores and J.A. Daròs (2008). Monomeric linear RNA of Citrus exocortis viroid resulting from processing in vivo has 5'-phosphomonoester and 3'-hydroxyl termini: implications for the RNase and RNA ligase involved in replication. Journal of Virology 82: 10321-10325. PubMed
  53. Marqués J., Z.G.N. Fadda, N. Duran-Vila, R. Flores, J.M. Bové and J.A. Daròs (2008). A set of novel RNAs transcribed from the chloroplast genome accumulates in date palm leaflets affected by brittle leaf disease. Phytopathology 98: 337-344. PubMed
  54. Gas M.E., C. Hernández, R. Flores and J.A. Daròs (2007). Processing of nuclear viroids in vivo: an interplay between RNA conformations. PLoS Pathogens 3: 1813-1826. PubMed
  55. Eiras M., E.W. Kitajima, R. Flores and J.A. Daròs (2007). Existence in vivo of the loop E motif in potato spindle tuber viroid RNA. Archives of Virology 152: 1389-1393. PubMed
  56. Molina-Serrano D., L. Suay, M.L. Salvador, R. Flores and J.A. Daròs (2007). Processing of RNAs of the family Avsunviroidae in Chlamydomonas reinhardtii chloroplasts. Journal of Virology 81: 4363-4366. PubMed